Bd facsaria user guide bd facsaria ii has three lasers. However, you can create new modes and then select them from the precision mode menu. Manual of standard operating procedures uw pathology. Micromanipulators for manual cell picking typically consist of an inverted microscope combined with micropipettes movable through motorized mechanical stages. A biosafety cabinetenclosed highspeed cell sorting instrument equipped with 375nm, 405nm, 445nm, 488nm, 561nm, and 640nm lasers.
Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria user s guide. Facsaria protocol littman lab version 040208 elke kurz nicolas manel. Each laser has its own filters for detectiong emmision spectrum. A copy is at the end of this manual, for the new user to be certified to enter the core or work independently in the facility. Refer to the sys refer to the tlink console user manual for details. This guide shows a workflow that uses application settings. The heart of this cell sorting revolution is the true fixedalignment flow cell, gelcoupled to the collection optics. Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they. Patented bd facs accudrop technology assists the user to see the best drop.
Bd life sciences diagnostic systems qcpi manuals bd. The software interface that comes with it is not exactly userfriendly and the manual is too expansive and too general. Page 1 use this quick reference guide once you are familiar with the procedures in the bd facsaria ii user s guide. Starting up the cytometer follow these steps to start up your bd facsaria cytometer.
The properties measured include a particles relative size, relative granularity or internal complexity, and relative. Turn on the stream and allow time for it to stabilize. Text and keyboard conventions are shown in table 2. The bd facsaria iii is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that has led to the unparalleled success of the bd facsaria.
For applicatio nspecific instructions, refer to the individual application guides and information supplied with the reagents. Bd facsaria iii cell sorter user s guides and manuals. The bd facsaria users guide assumes you have a working knowledge of basic. Analysis and sorting of cells with facsaria ii flow cytometer. Micropipettes are made of ultrathin glass capillaries coupled to an aspiration and dispensation unit.
Cytometry facsaria not responding purdue university. If you decide to go with the aria iii, just make sure that you get the bigger tip and a chiller. The bd facsaria iii sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary easeofuse that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. Bd facsariaii has fixedalignment cuvette flow cell, which allows achieving superior fluorescence sensitivity. Bd facsaria ii quick reference manual pdf download. It accommodates up to six aircooled lasers and eighteen fluorescent detectors. We have 1 bd facsaria manual available for free pdf download. Bd biosciences facsdiva software features bd facsdiva. Bd facsaria ii users guide the bd facsaria ii cytometer uses lowpowered, aircooled and solidstate lasers that do not have special power or cooling requirements. Cytometry facsaria not responding steve jungers sejungers411 at wed nov 22 14. A mask is a region of the stream which is monitored for the presence of cells of interest defined by bd biosciences facsaria training manual. For more details, refer to chapter 4 in the user s guide. Cells can be sorted into tubes or multiwell plates.
Bd the bd facsaria iii users guide assumes you have a working knowledge of. Pathology flow cytometry facility sop university of washington page 2 table of contents i. Fsc, ssc, 2 blue or 445nm, 5 yellowgreen, 6 violet, and 3 red. Export in a subfolder named after the username you use to. If you are a registered library contact, please login with your support.
Samples preparation filter sample before sorting 70 or 100 nm filter dont dilute sample too much, as more dense as faster the sort. Bd facsaria cellsorting system from bd biosciences. Bd facsaria fusion manual 227 amici del golden retriever. Check that the air line and the blue fluidics line is connected to the sheath tank. Check that the sheath tank is full and the waste tank is empty. Implemented 09192007 102007 ciiiv aar manual of standards and recommended practices specifications for tank cars section ciii table of contents see back of book for aar forms for reproduction by the proponents. It is the responsibility of the buyeruser to ensure that all added electronic files including software and transport media are virus free. Facsaria sorp standard operation protocol basic operation. The following figure shows the steps for daily workflow using the bd facsaria users guide assumes you have a working knowledge of basic. Bd lsrfortessa and bd facsaria iii openbis documentation. Ensure that both vent caps are tightened before installing.
Bd facsaria users guide national center for xray tomography. Fusion nozzle are label ms aria ii nozzle are label m do not switch nozzle between instruments. Keep this safety information available for future reference and for new users. Julie julie nelson flow cytometry core facility center for tropical and emerging global disease university of georgia jnelson at uga. Refer to figure 12 in the system iii installation manual. Operate the instrument only as directed in the users guide. For more details, refer to chapter 4 in the users guide. Bd facsaria quick reference guide use this quick reference guide once you are familiar with the procedures in the bd facsaria users guide. A bd facsaria iii cytometer report includes the following additional information. I will add some more information to the johns answer. Laser safety lasers or laser systems emit intense, coherent electromagnetic radiation that.
Bd facsaria iii system is even more powerful, dependable, and easy to use. Bd facsaria iii users guide core facility flow cytometry. The bd facsaria iii users guide assumes you have a bd facsdiva software quick reference guide for bd facsaria cell sorters. The bd facsaria is new generation benchtop highspeed flow cytometry cell sorter. See determining the drop delay manual method on page 172 for more information. Bd facsaria fusion fluorescence activated cell sorter. Facsaria fusion sorp quick reference guide childrens. Fx programming manual ii jy992d88101 this manual contains instruction explanations for the fx1s, fx1n, fx2n and fx2nc series programmable controllers. The new bd facsaria ii cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. Manual of standard operating procedures user registration and safe working practices bd facsaria iii cell sorting in h581c. Users manual hardware edition jy997d28701 this manual contains hardware explanations of wiring, installation and specifications for the fx3uc series programmable controllers. Table 1 lists symbols that are used in this manual or on safety labels to alert you to a potential hazard. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the endportion of the breaking stream can still be observed.
That means the fluorochromes pecy7 and apccy7 though emit in the same range in 78060, but since they excited essentially by different lasers, the emmision spectra will not interfer each other. Masks divide cells into 32 equal segments, and choosing different types of available masks determines how drops will be deflected if a sorting conflict occurs bd biosciences facsaria training manual. Setup on page 1 in the bd facsdiva software reference manual. Learn about bd life sciences diagnostic systems qcpi manuals. Adjust the frequency freq between 27 and 30 to identify the highest breakoff, where the end. The fluidics and optical systems are precisely integrated to maximize signal. Bd serves and supports our customers with full offerings beyond our individual products, enabling integrated solutions across healthcare systems to comprehensively improve care and lower costs. Add 250 ml of bleach to the empty waste tank if necessary. You will want to check the plates to make sure they are dry and clean before you turn on the stream. Crossinstrument and crosssite standardization using bd facsdiva softwares custom application settings. Facsaria ii users guide and page number for additional information. Starting up the cytometer follow these steps to start up your bd facsaria ii cytometer. Ensure the flow cell access door is raised and the sort chamber door is open.
Analysis and sorting of cells with facsaria ii flow cytometer 10. Please login to for documentation and training materials. For a full description of the cytometer status report, see the bd facsdiva software reference manual. New worksheet features create headers and footers on the worksheet, including cytometer name, experiment name, user name, date and time, bd facsdiva software. Several default entries are provided that may be utilized until they are deleted andor until new initials and identification codes are entered into the system. Bd facsaria quick reference guide biosciences area. With its fixedoptics design and digital electronics, the bd facsaria iii flow cytometer enables multicolor analysis of up to 18 fluorescent and two scatter options at a time. Stream breakoff and droplet formation 100m nozzle a. Bd facsariaii delivers highspeed multicolor sorting with digital acquisition rates of up to 20,000 eventssecond. Sims iii log in screen test users must enter their user name and password.
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