Genetically diverse uropathogenic escherichia coli adopt a. We have completed the genome sequence of the highly virulent upec strain cft073, isolated. Pathogenic escherichia coli an overview sciencedirect. Definition of uropathogenic escherichia coli in the dictionary. Uropathogenic escherichia coli upec cause impairment of. Escherichia coli migula castellani and chalmers atcc. Extraintestinal pathogenic escherichia coli expec, a heterogeneous group of e.
Pathogenomics of uropathogenic escherichia coli agarwal j. Epidemiology and prevention of escherichia coli o157. Typical and atypical enteropathogenic escherichia coli epec strains differ in several characteristics. However sometimes it causes extraintestinal infections such as urinary tract infections utis, in this case they are named uropathogenic e. A recent study combining genome hybridization and in. Biofilm formation in uropathogenic escherichia coli strains. Uropathogenic escherichia coli upec are a heterogeneous group of strains within the broader classification of extraintestinal pathogenic e. There is no core set of virulence factors shared by all upec. The bacteria can be found in soil or water and on surfaces that have been. Biofilm formation in uropathogenic escherichia coli. A common virulence genotype of upec 22 strains responsible for utis is yet to be defined, due to the large variation of virulence 23 factors observed in upec strains. Scientific committee on enteric infections and foodborne.
Uropathogenic escherichia coli mediated urinary tract infection volume. Soto, francesc marco, elisabet guiral and jordi vila. Virulence of strains may differ, and may be enhanced by antimicrobial resistance and biofilm formation, resulting in increased morbidity and recurrent infections. Pdf pathogenomics of uropathogenic escherichia coli. Uroplakin ia is the urothelial receptor for uropathogenic escherichia coli. Uropathogenic escherichia coli upec strains cause most uncomplicated. In the merged image in c factin is red, while upec is blue. Enterohemorrhagic escherichia coli ehec is a subset of pathogenic e. Uropathogenic escherichia coli mediated urinary tract. Relationship with urovirulence factors and antimicrobial resistance.
Uropathogenic escherichia coli mediated urinary tract infection. In vitro screening of multidrug resistance uropathogenic escherichia. The biology of the escherichia coli extracellular matrix. They live in the intestines of infected people and animals and are passed in the feces. Uropathogenic escherichia coli upec express extracellular, adhesintipped fibers that bind mucosal surfaces of the urinary tract. Inflammationinduced adhesinreceptor interaction provides. Recently it was discovered that uropathogenic escherichia coli, the primary causative agent of urinary tract infections, can invade and replicate within uroepithelial cells. Typical and atypical enteropathogenic escherichia coli. Upec strain cft073, derived from the clonal group o6.
Six pathotypes are associated with diarrhea diarrheagenic. Escherichia coli is the most common uropathogen involved in urinary tract infection uti. School of chemistry and molecular biosciences, building 76, university of queensland, brisbane qld 4072, australia. Strains of uropathogenic escherichia coli upec are the major cause of urinary tract infections. To determine the virulence factors produced by escherichia coli isolated from extraintestinal infections, to study the drug resistance pattern in e. Information and translations of uropathogenic escherichia coli in the most comprehensive dictionary definitions resource on the web. Upec expresses a multitude of virulence factors to break the inertia of the mucosal barrier. Porin channel triplets merge into single outlets in escherichia coli outer membranes.
H1, was originally isolated from the blood and urine of a woman diagnosed with acute pyelonephritis. Escherichia coli mediated urinary tract infections. Escherichia coli isolates that carry vat, fyua, chua, and yfcv. The adhesin that has been most closely associated with uropathogenic e.
The letter designation is derived from the ability of p fimbriae to bind specifically to the p blood group antigen which contains a dgalactosedgalactose residue. Background classification and evolution of uropathogenic e. Role of uropathogenic escherichia coli virulence factors. H7 infection purpose this paper provides an overview of escherichia coli o157. H7 infection, the global and local epidemiology, current health protection measures in hong kong, and recommendations for further prevention and control of the disease. Schembri2,3 1school of medical sciences, centre for medicine and oral health, griffith university, southport, australia 2australian infectious diseases research centre and 3school of.
The aim of this study was to evaluate the in vitro biofilm forming capacity of e. Pdf urinary tract colonization is enhanced by a plasmid. Typical epec, a leading cause of infantile diarrhea in developing countries, is rare in industrialized countries, where atypical epec seems to be a more important cause of diarrhea. Uropathogenic escherichia coli outer membrane antigens expressed during urinary tract infection. Escherichia coli plays an important role as a member of the gut microbiota. Extensive mosaic structure revealed by the complete genome sequence of uropathogenic escherichia coli. Bacterial adheren ce to and colonization of the urinary tract by upec strains are mediated by the expression of several types of fimbrial and nonfrimbrial ad hesins 21, 23, like most adhesions, it has been difficult to precisely define the role of any parti cular adhesine due to overlapping function. First described in 1885, e coli has become recognized as both a harmless commensal and a versatile pathogen. Pdf molecular epidemiology of escherichia coli mediated urinary. Enteropathogenic escherichia coli harvard catalyst. Virulence factors and drug resistance in escherichia coli. Upec possess a variety of virulence factors vfs, which the organism uses to attach. Escherichia coli which accounts for 90% of community acquired and 50% of hospital acquired utis. Escherichia coli is the major factor of communityacquired uti.
Pdf uropathogenic escherichia coli mediated urinary. The primary etiologic agents associated with utis are strains of uropathogenic escherichia coli upec. Isolation of trna from uropathogenic escherichia coli. Transcriptome of uropathogenic escherichia coli during. Dosage effect on uropathogenic escherichia coli antiadhesion activity in urine following consumption of cranberry powder standardized for proanthocyanidin content.
Pdf communityacquired urinary tract infections utis are a major. Uropathogenic escherichia coli upec is a causative agent in the vast majority of urinary tract infections utis, including cystitis and pyelonephritis, and infectious complications, which may result in acute renal failure in healthy individuals as well as in renal transplant patients. Escherichia coli is a commensal of the human intestine. Virulence factors in fecal escherichia coli from humans. Among the common uropathogens associated to utis development, uropathogenic escherichia coli upec is. Urinary tract infections caused by escherichia coli are the most common bacterial infections.
Makrina totsika, danilo gomes moriel, adi idris, benjamin a. Hemorrhagic colitis occasionally progresses to hemolytic uremic syndrome hus, an important cause of acute renal failure. Marrs, lixin zhang, betsy foxman department of epidemiology, university of michigan school of public health, 109 observatory street, ann arbor, mi 481092029, usa. Disclosed herein are various genes that can be included in immunogenic compositions specific for pathogenic e. Urinary tract infections utis are one of the most common pathological conditions in both community and hospital settings. Uropathogenic escherichia coli superinfection enhances the severity of mouse bladder infection. Certain strains cause diarrhea, and all can cause infection when they invade sterile sites eg, the urinary tract. Most upec belong to b2 and d phylogroups and can be classified based on the o lipopolysaccharide serotype into. Escherichia coli infections msd manual professional edition. Uropathogenic strains of escherichia coli upec are the most common cause of nonhospitalacquired urinary tract infections, responsible for 7090% of the 7 million cases of acute cystitis and 250,000 cases of pyelonephritis reported annually in the united states. These at proteins are associated with a number of serious, possibly life.
Uropathogenic escherichia coli upec cause impairment of spermatogenesis by inducing programmed necrosis inaugural dissertation submitted to the faculty of medicine in partial fulfillment of the requirements for the phddegree of the faculty of medicine justus liebig university giessen by lu, yongning guangxi, china giessen 2011. Uropathogenic escherichia coli upec are the most important group of microorganisms responsible for urinary tract infection. Although often categorized as extracellular pathogens, upec can in fact invade a number of host cell types. Virulence factors and innovative strategies for the treatment and.
The genes are from uropathogenic strains but are absent from nonpathogenic strains, and their encoded proteins have cellular locations which render them accessible to. Finally, a neighborjoining dendrogram was constructed in mega, version 5. The gramnegative bacterium escherichia coli is the most numerous aerobic commensal inhabitant of the large intestine. Persistence of uropathogenic escherichia coli in the face. Uropathogenic escherichia coli how is uropathogenic. Cn102675432a immunogens from uropathogenic escherichia. Urinary tract infections utis currently rank among the most prevalent of infectious diseases worldwide, with chronic and recurrent infections being especially problematic. Uropathogenic escherichia coli upec sequencing project. Virulence factors in fecal escherichia coli from humans and animals stephen hill university of guelph, 2012. These bacteria are found worldwide thus including new brunswick. Enteropathogenic escherichia coli is a descriptor in the national library of medicines controlled vocabulary thesaurus, mesh medical subject headings. Uropathogenic escherichia coli upec are phenotypically and genotypically.
Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity. This microorganism has virulence and antibiotic resistance, namely to. Pyelonephritogenic escherichia coli and killing of cultured human renal proximal tubular epithelial cells. Escherichia coli are gramnegative bacteria that inhabit the gastrointestinal tract. Escherichia coli, a facultatively anaerobic gramnegative bacillus, is a major component of the normal intestinal flora and is ubiquitous in the human environment. It has been estimated that about 150 million people worldwide develop uti each year, with high social costs in terms of hospitalizations and medical expenses. A strategy for generating extracellular fiberlike aggregates. The technical roadmap for extracellular secretion and onlinecleavage of antimicrobial peptides on the surface of escherichia coli. Uropathogenic escherichia coli upec is by far the most common etiological agent of all utis.
Within the expec classification are the uropathogenic escherichia coli strains that are able to cause pyelonephritis, prostatitis, and catheterassociated urinary tract infections utis as well as communityacquired and nosocomial utis in. The j biological c 2005 by the american society for. Pdf urinary tract infection uti is one of the most frequently acquired. Thirty uropathogenic li isolates were isolated from hospitalized and non hospitalized patients, complaining of urinary tract infections, of alkadhymia teaching hospital their ages ranging from 6. Request pdf uropathogenic escherichia coli it is noted that urinary tract is considered the most common sites of bacterial infection, and li is the most common infecting agent at this. The pathogenesis of escherichia coli urinary tract infection.
Uropathogenic escherichia coli virulence and innate immune. Uropathogenic escherichia coli upec is responsible for 80% of community acquired utis. Occurrence of genes encoding enterotoxins in uropathogenic. Uropathogenic escherichia coli upec have several virulent determinants which enable them to colonize the bladder mucosa and injure it, leading to inflammatory changes. Ulett1, makrina totsika2,3, kolja schaale2,4, alison j. Sat, the secreted autotransporter toxin of uropathogenic escherichia coli is a vacuolating cytotoxin for bladder and kidney epithelial cells.
1430 355 69 694 1307 160 1569 471 164 1064 319 593 1479 1294 1006 1047 1577 1360 969 444 1351 1610 374 1222 42 1574 778 1579 36 214 972 1444 1 1035 488 708 933 813 1225 601